To describe the law of platoon dispersion under the condition of heterogeneous bicycle traffic flow adequately, theoretical support is provided for urban bicycle signal coordination control，bicycle microscopic traffic simulation and traffic flow prediction. The characteristics of cycle’s travel time distribution in heterogeneous bicycle traffic flow is considered. The truncated Gaussian distribution is used to fit vehicle’s travel time distribution. Based on this, the investigated dispersion characteristics of urban heterogeneous cycle’s travel time is subject to truncated gaussian distribution. Later, the relationship between the arrival flow rate of the downstream intersection and the depart flow rate of the upstream intersection is analyzed using the proposed model by field collected data, with comparison to those of Robertson model and the actual data. The results show that the proposed model can better describe the law of dispersion in heterogeneous bicycle traffic flow, and the mean squared error of prediction is reduced by about 22.53%, compared with Robertson model.