Fatigue damage of long-span bridge suspenders is a serious problem under long-term environmental erosion and repeated vehicle loads. In order to accurately evaluate the service condition of parallel steel wire suspenders, the relationship between of fatigue crack growth model parameters and stress ratio was fitted based on the result of fatigue crack growth test of high strength steel wires under different stress ratios. The stress concentration effect caused by pitting corrosion was then incorporated into the stress intensity factor model. The fatigue crack growth analysis model was integrated from the equivalent initial crack size to a critical crack length to predict the fatigue life of a single steel wire. The fatigue life of the entire suspender can be assessed considering the relevance of fracture steel wire and the stress redistribution of suspender after the fracture of steel wires. Moreover, the effects of steel wire fracture ratio, frequency of vehicle load and corrosion pit growth rate on the service life of suspender was analyzed. This study may provide a reference for the safety evaluation and operational maintenance of existing long-span bridge suspenders.