In order to reduce the pollution of expressway tunnel grouting construction in Karst areas，the effect of various green admixtures was analyzed，and the optimal parameters of green admixture at different setting time was analyzed based on the Darwinian genetic algorithm and multiple linear regression method.The test results show that，sodium gluconate，calcium formate and sodium gluconate are considered as the most suitable green grouting material of retarder，early strength and water reducer agent， respectively. When the initial coagulation time is equal or lesser than 30 min，and the final coagulation time is equal or lesser than 33 min，the optimal mixing amount is 2.564% calcium formate and 0.039% polycarboxylic acid，without sodium gluconate. When the initial coagulation time is 30~60 min，and the final coagulation time is 33~65 min，the optimal mixing amount is 2.48% calcium formate and 0.117% polycarboxylic acid，respectively，without sodium gluconate. When the initial coagulation time exceeds 60 min，and the final coagulation time exceeds 65 min，the optimal incorporation is 0.022% sodium gluconate，1.58% calcium formate，and 0.140% polycarboxylate，respectively.