In order to study optimization of vehicle guidance signs after the significant increase of the ETC vehicles on highway，the efficacy experimental on three toll station lane guidance sign schemes (the unoptimized traditional scheme，arrow and the MTC guidance scheme，and voice and the MTC guidance scheme)was carried out in this study. The data indicators of drivers' lane changing behavior was collected. The differences of drivers' lane changing behaviors under different proactive notice guidance sign schemes were compared using single factor analysis of variance . The experimental results show that the response time can been reduced significantly using the optimized guidance sign. The lane change decision will be guided to make in advance，reducing the conflict between MTC vehicles and mainline ETC vehicles in front of the toll station. Therefore，the lane change process will be done steady and safely.